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static void Main(string[] args)
{
    Bitmap bmp = new Bitmap("d:/tmp/screenshot.png");
    Bitmap gray;
    Stopwatch sw = new Stopwatch();

    sw.Start();
    gray = ColorToGrayscale(bmp);
    sw.Stop();

    gray.Save("d:/tmp/screenshot_gray.png");

    Console.WriteLine(sw.Elapsed.TotalMilliseconds);

    Console.ReadKey();
}

/// <summary>
/// Converts a bitmap into an 8-bit grayscale bitmap
/// </summary>
public static Bitmap ColorToGrayscale(Bitmap bmp)
{
    int w = bmp.Width,
        h = bmp.Height,
        r, ic, oc, bmpStride, outputStride, bytesPerPixel;
    PixelFormat pfIn = bmp.PixelFormat;
    ColorPalette palette;
    Bitmap output;
    BitmapData bmpData, outputData;

    //Create the new bitmap
    output = new Bitmap(w, h, PixelFormat.Format8bppIndexed);

    //Build a grayscale color Palette
    palette = output.Palette;
    for (int i = 0; i < 256; i++)
    {
        Color tmp = Color.FromArgb(255, i, i, i);
        palette.Entries[ i ] = Color.FromArgb(255, i, i, i);
    }
    output.Palette = palette;

    //No need to convert formats if already in 8 bit
    if (pfIn == PixelFormat.Format8bppIndexed)
    {
        output = (Bitmap)bmp.Clone();

        //Make sure the palette is a grayscale palette and not some other
        //8-bit indexed palette
        output.Palette = palette;

        return output;
    }

    //Get the number of bytes per pixel
    switch (pfIn)
    {
       case PixelFormat.Format24bppRgb: bytesPerPixel = 3; break;
       case PixelFormat.Format32bppArgb: bytesPerPixel = 4; break;
      
case PixelFormat.Format32bppRgb: bytesPerPixel = 4; break;
       default: throw new InvalidOperationException("Image format not supported");
    }

    //Lock the images
    bmpData = bmp.LockBits(new Rectangle(0, 0, w, h), ImageLockMode.ReadOnly,
                           pfIn);

    outputData = output.LockBits(new Rectangle(0, 0, w, h), ImageLockMode.WriteOnly,
                                 PixelFormat.Format8bppIndexed);

    bmpStride = bmpData.Stride;
    outputStride = outputData.Stride;

    //Traverse each pixel of the image
    unsafe
    {
        byte* bmpPtr = (byte*)bmpData.Scan0.ToPointer(),
        outputPtr = (byte*)outputData.Scan0.ToPointer();

        if (bytesPerPixel == 3)
        {
            //Convert the pixel to it's luminance using the formula:
            // L = .299*R + .587*G + .114*B
            //Note that ic is the input column and oc is the output column
            for (r = 0; r < h; r++)
                for (ic = oc = 0; oc < w; ic += 3, ++oc)
                    outputPtr[r * outputStride + oc] = (byte)(int)
                        (0.299f * bmpPtr[r * bmpStride + ic] +

                         0.587f * bmpPtr[r * bmpStride + ic + 1] +
                         0.114f * bmpPtr[r * bmpStride + ic + 2]);
        }
        else //bytesPerPixel == 4
        {
            //Convert the pixel to it's luminance using the formula:
            // L = alpha * (.299*R + .587*G + .114*B)
            //Note that ic is the input column and oc is the output column
            for (r = 0; r < h; r++)
                for (ic = oc = 0; oc < w; ic += 4, ++oc)
                    outputPtr[r * outputStride + oc] = (byte)(int)
                        ((bmpPtr[r * bmpStride + ic] / 255.0f) *
                        (0.299f * bmpPtr[r * bmpStride + ic + 1] +
                         0.587f * bmpPtr[r * bmpStride + ic + 2] +
                         0.114f * bmpPtr[r * bmpStride + ic + 3]));
        }
    }

    //Unlock the images
    bmp.UnlockBits(bmpData);
    output.UnlockBits(outputData);

    return output;
}

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